LOCUS       40924_48348        3901 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pZE1PA1lacO-1 luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Independent and tight regulation of transcriptional units in 
            Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 
            regulatory elements
  JOURNAL   Nucleic Acids Res. 25 (6), 1203-1210 (1997)
  PUBMED    9092630
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Lutz R, Bujard H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-1996) Zentrum fuer Molekulare Biologie, University
            of Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 3901)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3901)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997"; volume: "25"; issue: "6"; pages: 
            "1203-1210"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-AUG-1996) Zentrum fuer Molekulare Biologie, University of 
            Heidelberg, Im Neuenheimer Feld 282, Heidelberg 69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..48
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             100..111
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             118..1767
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      1786..1872
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2036..2624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2712..2806)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2839..3696)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3697..3801)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatg atacttagat
       61 tcaattgtga gcggataaca atttcacaca gaattcatta aagaggagaa aggtaccatg
      121 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctaga ggatggaacc
      181 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
      241 tttacagatg cacatatcga ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt
      301 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
      361 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
      421 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gaacatttcg
      481 cagcctaccg tagtgtttgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
      541 aaattaccaa taatccagaa aattattatc atggattcta aaacggatta ccagggattt
      601 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
      661 gtaccagagt cctttgatcg tgacaaaaca attgcactga taatgaactc ctctggatct
      721 actgggttac ctaagggtgt ggcccttccg catagaactg cctgcgtcag attctcgcat
      781 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
      841 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
      901 gtcgtcttaa tgtatagatt tgaagaagag ctgtttttac gatcccttca ggattacaaa
      961 attcaaagtg cgttgctagt accaacccta ttttcattct tcgccaaaag cactctgatt
     1021 gacaaatacg atttatctaa tttacacgaa attgcttctg ggggcgcacc tctttcgaaa
     1081 gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca aggatatggg
     1141 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
     1201 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
     1261 ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat gtccggttat
     1321 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
     1381 gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt gaagtcttta
     1441 attaaataca aaggatacca ggtggccccc gctgaattgg agtcgatatt gttacaacac
     1501 cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg tgaacttccc
     1561 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
     1621 gtcgccagtc aagtaacaac cgccaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
     1681 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
     1741 gccaagaagg gcggaaagtc caaattgtaa tgactctaga ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1861 agtaggacaa atccgccgcc ctagacctag gcgttcggct gcggcgagcg gtatcagctc
     1921 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2221 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2461 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2701 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     2761 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta
     2821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     3841 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     3901 c
//
